Figure 6

Figure 6

(A) qPCR of GPR12 mRNA expression in oocytes (6–8 mm Oo) of chicken 6–8 mm follicles and granulosa cells of 6–8 mm (6–8 mm GC), F5 (F5-GC) and F1 follicles (F1-GC). (B,C) Predominant expression of BMP15 (B) and GDF9 (C) mRNA in 6–8 mm GC, 6–8 mm Oo, F5-GC and F1-GC. In graphs A–C, different letter indicates the statical difference between two groups. (D) RT-PCR detection of GPR12 mRNA expressed in chicken 6–8 mm GC, 6–8 mm Oo, F5-GC, and F1-GC. The GPR12 band intensity is the strongest in 6–8 mm Oo, and its signal in GC cells decreases along follicle development. No PCR band was detected in all reverse transcription (RT)-negative controls. In graphs A–D, each data point represents the mean ± SEM of 4 replicates (N = 4). (E) The constitutive activity of GPR12 in cultured 6 mm GC transfected with cGPR12 plasmid as detected by dual-luciferase reporter assay. Each data point represents the mean ± SEM of four replicates (N = 4). *** p < 0.001 vs. control.