|
Fig. 3 Expression of phoenixin/smim20 and of PNX-like immunoreactivity in the adult hypothalamus. (A, A′, B, B′: transverse cryosection) (A, A′) phoenixin/smim20 is expressed in the magnocellular nucleus (PM) of the POA; (A′) Higher magnification of boxed area in A. (B) PNX-like immunoreactivity in cells distributed adjacent to the diencephalic ventricle (DiV) and in the magnocellular pre-optic nucleus (PM) and parvocellular pre-optic nucleus (PPp). (B′) Higher magnification of boxed area in B. (C, C′) Sagittal sections, anterior to the left, labeled with anti-PNX antibody. (C) Immunolabeling of cells in the POA. (C′) Higher magnification view of area boxed in C. (D-D″) Transverse cryosections showing fluorescently labeled PNX-like immunoreactivity (red, D, D′) and ant-Zrf1 (green, D, D″) in the posterior parvocellular preoptic nucleus PPp (see Fig. 2F). (E) In situ hybridization showing phoenixin/smim20 expression in the same region (arrow D-D″, E; transverse cryosection). Abbreviations: PM: magnocellular pre-optic nucleus; PPp: parvocellular pre-optic nucleus, posterior part; SC: suprachiasmatic nucleus, ON optic nerve. A-E. All images are from 20 μm cryostat cross-sections of adult brain. Scale bars: A, B, C = 50 μm; A′, B′, C’ = 25 μm. D-D″, E = 30 μm.
Reprinted from Gene expression patterns : GEP, 39, Ceriani, R., Calfún, C., Whitlock, K.E., phoenixin(smim20), a gene coding for a novel reproductive ligand, is expressed in the brain of adult zebrafish, 119164, Copyright (2020) with permission from Elsevier. Full text @ Gene Expr. Patterns