Figure 8

Figure 8

Fisetin inhibits LPS-induced inflammation and endotoxic shock by stimulating the β-catenin signaling pathway in zebrafish larvae. Zebrafish larvae at 3 dpf were pretreated with 10 µM FH535 for 2 h prior to LPS (2 nL of 0.5 mg/mL) microinjection. Then, the larvae were grown in E3 media containing 400 μM fisetin. (A) Neutral red-stained macrophages are shown at 18 hpi. (B) Sudan black-stained neutrophils were observed at 18 hpi. (C) At 18 hpi, 10 zebrafish from each condition were collected and the expression of iNOS and COX-2a was detected by RT-PCR. GAPDH was used as an internal control. Each value indicates the mean ± standard error median (SEM) and is representative of the results obtained from three independent experiments. Significant differences among the groups were determined using one-way ANOVA with Bonferroni correction (***p < 0.001, **p < 0.005, and *p < 0.01).