ZFIN Image: Cai et al., 2021, Fig. 5

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Figure Caption

Fig. 5 DNA-binding analysis of recombinant PcDp1 protein by EMSA. The purified recombinant PcDp1 protein was incubated with the DIG-labeled probe containing wild-type E2F binding site (wE2F), mutant binding E2F site (mE2F), or competition binding E2F site (cE2F). Complexes were separated on an 8% native PAGE and transferred onto a nylon+ membrane. Lane 1: negative group (without PcDp1 protein); Lane 2: wild E2F site group (PcDp1 protein with wild biotinylated E2F site); Lane 3: mutant E2F site group (PcDp1 protein with wild and mutant biotinylated E2F site); Lane 4: competition E2F site group (PcDp1 protein with wild and competition biotinylated E2F site).

Acknowledgments

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