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Figure 1—figure supplement 2. Evaluation of BirA2-GFP-CAAX localization and biotinylation.

(A) BirA2-GFP-CAAX is expressed in cardiomyocytes via the cmlc2 promoter. (B) BirA2-GFP-CAAX is localized to the cell membrane. Membrane staining with wheat germ agglutinin showed colocalization with the BirA2-GFP-CAAX signal. Scale bar: 10 µm. (C–G) Immunofluorescence images of sections of ventricles with native BirA2-GFP-CAAX signal stained for DAPI. Animals were injected intraperitoneally with biotin for 1, 2, 3, or 4 days. dpt: days post treatment. Scale bar: 50 µm.

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