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Figure 8 Qx28 modulates NF-κB canonical pathway. Representative immunoblots and quantitative data for IκBα. MEFs were treated for 16 hours with the indicated compounds, then processed for Western blot analysis. Membranes were stripped and reprobed for β-actin as a loading control. TNF-α (10 ng/mL) was used as a positive control for NF-κB canonical pathway activation. Quantification of the bands was performed using ImageJ. Results are expressed as mean ± SEM from 3 independent experiments. Statistical analysis: 2-tailed Student’s t test, *P < 0.05, **P < 0.01, ****P < 0.0001 versus the corresponding ototoxin alone. TNF-α treatment was compared with Qx28 10 μM treatment. Cartoon: signaling molecules involved in the NF-κB canonical pathway.