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Figure 4 Effect of AI-EtE on development and survival of zebrafish embryos at different time points. The effects of AI-EtE at various doses (0, 5, 10, 12.5, 25, 50, 75, 100, 200 and 400 mg/L) on defects during development (A), lethality (B), and hatching percentage (C) of zebrafish embryos at 24, 48, 72 and 92 hpf, respectively, are presented as dose-response curves. Representative images of 96 hpf zebrafish larvae presenting various developmental defects upon AI-EtE exposure at different doses, (D) 0 mg/L (negative control), (E) 25 mg/L, (F) 50 mg/L, (G) 75 mg/L, (H) 100 mg/L, (I) 200 mg/L, and (J) 400 mg/L. Illustrated defects on larvae include yolk-sac oedema (e), haemovascular defect (h), and necrosis (n) as indicated with arrows.