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Figure 6 Sodium lactate attenuates AHF. AHF in zebrafish embryos was induced by (A) 10 μM AA from 24–72 hpf or (B) 5 μM AA from 24–96 hpf, as lower concentration AA takes a longer time to induce severe heart failure phenotypes. Sodium lactate was added to test its AHF attenuation ability at two concentrations (L, 1.5 mM; H, 15 mM). A2-4-2-4 was set up at two concentrations as well (L: 1:1000 dilution lactate; H: 1:300 dilution). Several positive compounds, NS398, MEK-I and C25, were set up for comparison.
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