ZFIN Image: Haege et al., 2021, Figure 3

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Figure Caption

Figure 3 Chemical analyses for A2-4-2-4. A2-4-2-4 was analyzed using NMR and ESIMS. (A) The 1H-NMR graph indicates a methyl group (δH 1.3, d, J = 7.0 Hz) and a methane group (δH 4.16, q, J = 7.0 Hz) in A2-4-2-4. (B) The ¹3C-NMR indicates a carboxyl group (δC 182.5), a hydroxyl group (δH 68.6), and a methyl group (δH 20.4) in the major compound in A2-4-2-4. (C) The 2-dimensional NMR, using heteronuclear multiple bond correlation, further confirmed that the major compound in A2-4-2-4 is lactate. (D) ESIMS analysis revealed that the molecular weight of the major compound in A2-4-2-4 is 89.0233 (C₃H₆O₃).

Acknowledgments

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