ZFIN Image: Acin-Perez et al., 2020, J

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Figure Caption

J Mitochondrial content quantification using MTDR

A

Fluorescence in freshly isolated mouse liver mitochondria under the indicated treatments and stained with MTDR and MTR. Fluorescence was normalized by protein and referred as percentage of untreated samples.

B

MTDR fluorescence in frozen liver mitochondria and homogenate at the indicated concentration. MTDR fluorescence correlates with protein amount but it saturates at higher protein levels.

C

Linear correlation between MTDR fluorescence and protein abundance in frozen liver mitochondria and homogenate, when the system is not saturated. For this correlation, saturation values have been excluded and only linear range values have been considered.

D

MTDR Operetta imaging in frozen liver mitochondria and homogenate at the indicated concentration.

E

Western blot for mitochondrial protein and actin in increasing concentrations of frozen liver mitochondria and homogenate as indicated.

F

Correlation between MTDR fluorescence (from B and C) and protein levels (from E) in frozen liver mitochondria and homogenate.

G

MTDR Operetta imaging in frozen homogenates of different tissues.

H

MTDR fluorescence in frozen tissue homogenate by microgram of sample.

I

Spectrophotometric citrate synthase activity measure by milligram of lysate.

J

Western blot and quantification of mitochondrial protein by actin (loading control) in tissue homogenates.

Data information: Biological replicates: (A), n = 4; (B–F), n = 5; and (G–J), n = 3. Every biological replicate represents the average of four technical replicates. Data are the mean ± SEM. Western blots illustrate a representative blot of three independent experiments. Source data are available online for this figure.

Acknowledgments

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