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Fig. 2 Tp1-positive BECs contribute to physiological hepatocyte homeostasis in a portion of zebrafish adults (A) Experimental scheme illustrating the stage of 4OHT treatment to triple transgenic line Tg(tp1:CreERT2; lfabp:loxP-STOP-loxP-DsRed; lfabp:Dendra2-NTR) from 5 dpf to 7 dpf and analysis at 1 and 2 years. (B) Live images showing the expression of Dendra2 and DsRed in the adult livers at 1 year. Co-immunostaining for Dendra2 and DsRed in the liver sections after DMSO and 4OHT treatment. (C) Fluorescence activating cell sorter (FACS) analysis showing the ratio of DsRed+ among Dendra2+ cells in DMSO- and 4OHT-treated livers. Quantification of the percentage of DsRed+ among Dendra2+ cells in DMSO- (n=3) and 4OHT-treated (no contribution, n=3; low contribution, n=4; high contribution, n=3) livers at 1 year. (D) Live images showing the expression of Dendra2 and DsRed in the adult livers at 2 years. Many clusters of DsRed+ cells among livers in 4OHT-treated groups. Co-immunostaining for Dendra2 and DsRed in the liver after DMSO and 4OHT treatment. (E) Fluorescence activating cell sorter (FACS) analysis showing the ratio of DsRed+ among Dendra2+ cells in DMSO- and 4OHT-treated livers. Quantification of the percentage of DsRed+ among Dendra2+ cells in DMSO- (n=5) and 4OHT-treated (no contribution, n=4; low contribution, n=5; high contribution, n=3) livers at 2 years. Numbers indicate the proportion of larvae exhibiting the expression shown. Asterisks indicate statistical significance: ∗p<0.05, ∗∗p<0.01, and p value was calculated by Student t tests. Scale bars: 100 μm. Data are represented as mean ±SEM.