Fig. 3.

Fig. 3.

Genomics and expression of ace, agtr, and nr3c2. (A) Conserved syntenies verify orthology of zebrafish ace to human ACE. (B) Expression of ace in a specific intestinal epithelial cell type in c152. (C) Clusters c101, c152, c168, and c214 are intestinal epithelial cell types (Farnsworth et al., 2020). (D) The age of each cell, as indicated in the insert (two replicates at each of three developmental stages), showed that ace is expressed in a 5 dpf intestinal cell type c152 that does not exist at 1dpf or 2dpf. (E) Double conserved synteny of zebrafish agtr1a and agtr1b to human AGTR1. (F) agtr1b was expressed only in endothelial cells (c88). Expression of agtr1a was not detected. (G) Conserved synteny of agtr2 and AGTR2 confirms orthology. (H) Expression of agtr2 was detected in c88 and c79 endothelial cells, including some in which agtr1b was detected. (I) Conserved syntenies for the aldosterone receptor gene nr3c2. (J) Expression detected for nr3c2 in intestinal epithelial clusters c168 and c101. (K-L) Expression of nr3c2 in the embryonic periderm (c0, c77) and in larval gills (c5).