FIGURE 3

FIGURE 3

Evaluation of control and patient‐specific hiPSC‐CM spontaneous contractile function by video microscopy. (A) Contraction cycles from phase‐contrast videos of control (left) and patient‐specific (right) hiPSC‐CM monolayers obtained using custom made software analysis. (B–J) Contraction characteristics of control (N = 229) and patient‐specific monolayers (N = 359). (B) Beat rate, (C) cellular displacement, (D) contraction velocity, (E) contraction duration from peak to 90% relaxation corrected with Bazett's formula, (F) area under the curve, (G) resting duration, (H) the percentage of videos demonstrating aberrant contractile events (control: 25% (278 areas out of 1097 total areas); patient specific: 35% (600 areas out of 1727 total areas)), (I) the percentage of recording spent in asynchrony and (J) the percentage of the video area concerned by aberrant events. (K) Spider chart illustrating the main differences observed between control (blue area) and patient‐specific (orange area) hiPSC‐CM. A “normal” value of 2 is attributed to the control parameter. Each evaluated parameter is compared to the control condition and fixed as higher (3) or lower (1) if statistically significantly different. Histograms represent the median (95% confidence interval). The horizontal grey area illustrates the difference between the control and the patient specific conditions. **p < 0.01 and ****p < 0.0001. Mann–Whitney test except for (H), Fisher's exact test