FIGURE 2

FIGURE 2

Calcification in single and double mutants of zebrafish ABCC6 paralogs. (A,B) Alizarin Red stained 10 dpf abcc6a^+/+ and abcc6a^–/– larvae. Ectopic calcification loci in mutants are denoted with arrowhead. (C) Quantification of calcified vertebrae in the 10 dpf progeny of abcc6a^+/– carriers, plotted by genotype. The results show a significant increase in the rate of calcification in the abcc6 mutants compared to their siblings (n = 65, significance levels calculated with Mann-Whitney test). (D) Quantification of calcified vertebrae in the 14 dpf progeny of abcc6b.1^+/– carriers, plotted by genotype. Results suggests that impairment of abcc6b.1 does not affect the rate of vertebral calcification (n = 72, significance levels calculated with Mann-Whitney test). (E) Quantification of calcified vertebrae in the 14 dpf progeny of abcc6a^+/–;abcc6b.1^+/– carriers, plotted by genotype (n = 68).