Figure 10

Figure 10

Delivery of DilC18 dye in GFAP::GFP-positive neural stem cells mediated by HDL intracerebroventricular injection. Three adult transgenic zebrafish (GFAP::GFP) were intracerebroventricularly injected with HDLs in which DilC18 dye was incorporated (fish were allowed to survive for 1 h 30 min). (A–D, I–L) Zebrafish brain sections at the junction through the olfactory bulbs (OB)/telencephalon (Tel) and through the anterior part of the preoptic area (PPa), showing DilC18 dye (red) along the ventricular layer where NSCs (green) are located. Note the uptake of the DilC18 dye (red) by NSCs (green) as shown by the yellow color in the merged pictures (see arrows). (E–H, M–P) Higher magnifications of the white boxes in (A–D, I–L), respectively. The arrows show colocalization of the dye with GFP, and arrowheads show the colocalization in neural stem cell processes. Scale bar = 700 μm (A, B), 140 μm (E–H), 85 μm (I–L), and 40 μm (M–P).