ZFIN Image: Sulliman et al., 2021, Figure 5

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Figure Caption

Figure 5

HDLs reach the brain microvasculature in zebrafish. ApoA-I immunohistochemistry (green) on zebrafish brain Sects. 1 h 30 min after intraperitoneal injection with 80 mg/kg of fluorescent HDL particles (red). Note that similar data were obtained with HDLs isolated from plasma (plHDL, n = 3) and with reconstituted HDLs (rHDL, n = 7). (A–L) ApoA-I staining (green) colocalizes with fluorescent HDLs (red) as clearly evidenced by the merged pictures and revealed blood vessel staining. (M–O) Confocal imaging of a transverse brain section from a Tg(fli1:EGFP) zebrafish expressing GFP in the endothelial cells from blood vessels⁵⁴. Note ApoA-I staining (red) detection in endothelial cells (green). The merge pictures (C, F, I, L, O) also show cell nuclei using DAPI counterstaining (blue). Scale bar = 50 μm.

Acknowledgments

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