ZFIN Image: Koopman et al., 2021, Fig. 4

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Figure Caption

Fig. 4 Tmem161b expression is specific to excitable cells and localizes to plasma membranes. A tmem161b BAC transgenic line, Tg(tmem161b^BAC:tmem161b-citrine), generated by fusing a Citrine cassette to the C terminus of the Tmem161b protein, permits analysis of the tmem161b expression pattern as well as protein localization. (A) At 5 dpf, Tg(tmem161b^BAC:tmem161b-citrine) transgenics show Tmem161b-Citrine expression in excitable cells, including cardiomyocytes (bracket), ionocytes (arrows), and neuromasts (arrowheads). (B) High-magnification view of ventricular cardiomyocytes at 5 dpf of Tg(tmem161b^BAC:tmem161b-citrine) embryos crossed to the Tg(myl7:cherry-caax) line (cardiomyocyte reporter) shows Tmem161b-Citrine localization at or near the plasma membrane of cardiomyocytes. (C) Imaging of ionocytes and neuromasts of Tg(tmem161b^BAC:tmem161b-citrine) at 5 dpf showing enrichment for Tmem161b-Citrine in these cell types. Ionocyte and neuromast identity are confirmed by staining with Mitotracker (magenta).

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Expression / Labeling details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA