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Publication: Han et al., 2021 - Deficiency in Neuroserpin Exacerbates CoCl₂ Induced Hypoxic Injury in the Zebrafish Model by Increased Oxidative Stress
All Figures: Figures for Han et al., 2021

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FIGURE 1

Generation of serpini1^−/− zebrafish via CRISPR-CAS9 technique. (A): Schematic representation of CRISPR/Cas9 target sites in zebrafish gene serpini1. The 19 bp target site is located on the exon4 of the serpini1 gene. (B): DNA sequencing results of the wild-type (WT) and homozygote serpini1^−/−. Serpini1^−/− mutation leads to five-nucleotide deletion in the fourth exon. (C): Protein comparison for WT and serpini1^−/− mutation. The serpini1^−/− caused a frameshift mutation, truncated from 415 aa into a 271 aa protein. (D). mRNA level of serpini1 in serpini1^−/− compared to WT. The data are presented as the mean ± SEM, N = 3 trails. Statistical analyses were performed with Student’s t-test, ****p < 0.0001 compared to WT.

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