Fig. 5.

Fig. 5.

Ectopic activation of endocardial Notch signaling in s411 embryos carrying the T757A PRKD2 mutation. Bright field (A,A′) and fluorescence (B,B′) analysis of s411 sibling and mutant embryos carrying the Tg(Tp1:mCherry) (pseudo-colored grey). Scale bars: 500 μm. Confocal analysis of 150 μm cardiac slices of s411 sibling and mutant embryos carrying the Tg(Tp1:mCherry) co-stained with 633-phalloidin (blue) (C,C′); Scale bar: 25 μm. Notch signaling is active in several tissues and organs throughout the embryo and restricted to the AV canal and OFT of wild-type embryos at 72 hpf (B,C). In s411 mutants, Notch signaling appears unaffected in the embryo (B′, white arrowheads) but remains active throughout the ventricular endocardium (B′, yellow arrowheads, C′). White and yellow arrows (C) indicate the Notch-positive cells at AV canal and OFT, respectively. AV, atrioventricular; OFT, outflow tract. n=10 in each of three independent experiments.