IMAGE

Fig. 2.

ID: ZDB-IMAGE-210320-29
Publication: Giardoglou et al., 2021 - A Zebrafish Forward Genetic Screen Identifies an Indispensible Threonine Residue in the Kinase Domain of PRKD2
All Figures: Figures for Giardoglou et al., 2021

Image

Figure Caption

Fig. 2.

The T757A substitution disrupts PRKD2 kinase activity. Lysates from HEK293 cells that heterologously overexpress wild-type or mutant forms of PKD1 and PKD2 were subjected to in vitro immuno-complex kinase assays (IVKAs) in the absence or presence of PS/PMA and immunoblot analysis was used to track PKD C-tail autophosphorylation as well as PKD1 phosphorylation of recombinant CREB (added as a heterologous substrate). All results were replicated in three separate experiments.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Biol. Open