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Fig. 2.

ID: ZDB-IMAGE-210320-29
Source: Figures for Giardoglou et al., 2021

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Figure Caption

Fig. 2.

The T757A substitution disrupts PRKD2 kinase activity. Lysates from HEK293 cells that heterologously overexpress wild-type or mutant forms of PKD1 and PKD2 were subjected to in vitro immuno-complex kinase assays (IVKAs) in the absence or presence of PS/PMA and immunoblot analysis was used to track PKD C-tail autophosphorylation as well as PKD1 phosphorylation of recombinant CREB (added as a heterologous substrate). All results were replicated in three separate experiments.

Acknowledgments

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