ZFIN Image: Guo et al., 2021, Fig. 2.

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Figure Caption

Fig. 2.

Identification of the efficiency of lhx9 MO in the zebrafish embryo. (A) Schematic diagram of lhx9 MO blocking Lhx9 mRNA splicing and protein translation. Exon 2 was deleted in the lhx9 transcript by MO, the LIM1 domain of Lhx9 protein was removed, and the LIM2 and HD domain can't be assembled because of the wrong amino acid sequences and the early stop codon. (B) RT-PCR confirming the effect of splice-blocking MO on lhx9 transcript at 24 hpf, 48 hpf, 60 hpf, 72 hpf, and 96 hpf. MO lanes (MO cDNA as the template) show two PCR bands, band 1 and 2 (736 bp). (C) Sequence alignment of band 1 (939 bp) and band 2 (736 bp).

Acknowledgments

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