FIGURE 7
GRb1 alleviates neutrophil infiltration of the liver during acute alcohol injury. Neutrophil fluorescence micrographs and quantification of the number of neutrophils in the liver (within the cycle). After alcohol modeling and GRb1 administration, zebrafish larvae with neutrophil-specific EGFP expression were observed and imaged using an Olympus U-HGLGPS fluorescence microscope (Tokyo, Japan). Figures are magnified as 20 and 50ⅹ. The number of neutrophils in the liver was counted using particle analysis in ImageJ software. Data are presented as the mean ± SEM (