GRB1 reduces the oxidative stress caused by alcohol in L-O2 cells. (A) Fluorescence micrographs of ROS in L-O2 cells. Figures are magnified as 200ⅹ. (B) Fluorescence micrographs of NA-8 in L-O2 cells. Figures are magnified as 200ⅹ. (C) Cellular fluorescence intensity of ROS as measured using a fluorescence microplate reader. (D) Cellular fluorescence intensity of GSH as measured using a fluorescence microplate reader. (E) MDA levels in L-O2 cells. (F) GSH levels in L-O2 cells. The data are displayed as the means ± SEM. p < 0.05 (#), p < 0.01 (##), and p < 0.001 (###) compared the control group; p < 0.05 (*), p < 0.01 (**), and p < 0.001 (***) compared with the 100 mM EtOH group.
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