Figure 2.

Figure 2.

Spatiotemporal expression patterns of trim46a in zebrafish embryos at 1 cell, sphere, shield, bud, 18 hpf and 24 hpf. trim46a transcripts were distributed in the precursor region of brain along the central nervous system. (A) Maternal messages of trim46a were present at 1-cell stage. (B) trim46a transcripts were abundant in the deep cell layer (DEL), enveloping layer (EVL) and I-YSL (yolk syncytial layer) at sphere stage. (C) trim46a transcripts were localized to the ventral & dorsal region at shield stage. (D) trim46a transcripts were abundant in the central nervous system at bud stage. (E and F) trim46a transcripts were evenly distributed in the brain region. (G and H) Lateral (G) and anterior (H) view of zebrafish embryos at 24 hpf; trim46a messages were restricted to the forebrain through the telencephalon, diencephalon, midbrain, MHB, cerebellum and rhombomere. All embryos were collected synchronously from WT zebrafish for WISH analysis at the corresponding stages. MHB, midbrain-hindbrain boundary; DMB, diencephalic-mesencephalic boundary; c, cerebellum; rh, rhombomere; t, telencephalon; m, midbrain; d, diencephalon; f, forebrain. (A–H) Scale bars: 50 μm.