ZFIN Image: Ranawakage et al., 2021, FIGURE 7

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Figure Caption

FIGURE 7

Expression of the tagged Sox3 protein. (A) Western blot and HiBiT blot analyses of the tagged Sox3 protein. Two-color Western blot analysis of sox3 knock-in embryos derived from the FLAGx3-50_#16 (a) and PAx3-50_#21 founders (b) with antibodies against Sox3, FLAG tag or PA tag, and α-tubulin. HiBiT blotting using an excessive amount of LgBiT protein and substrate to detect the C-terminal HiBiT peptide of the tagged Sox3 proteins (c). (B) Whole-mount immunohistochemical staining for the tagged Sox3 protein. Each primary antibody used to stain zebrafish is indicated below each image. Genotyping was conducted using PCR with genomic DNA prepared from the immunostained embryos after image acquisition.

Acknowledgments

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