ZFIN Image: Ranawakage et al., 2021, FIGURE 2

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Figure Caption

FIGURE 2

Cleavage efficiency of the selected crRNA. (A) Schematic illustration of the CRISPR-Cas9 ribonucleoprotein (RNP) complex and injection into a one-cell stage zebrafish embryo. (B) Candidate crRNA location and sequence. Double-strand break occurs 2 bases upstream from the stop codon for the selected crRNA. (C) Heteroduplex mobility assay (HMA) to evaluate the cleavage efficiency of crRNA. A 1.5 or 3 fmol RNP complex was microinjected, and genomic DNA was extracted from three pools of five embryos each at 1 dpf. Primers used for HMA are listed in Supplementary Table 1. (D) Percentage of indel mutations by Inference of CRISPR Edits (ICE) analysis.

Acknowledgments

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