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Fig. 1

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Publication: Pradhan et al., 2012 - Activation of NF-kappaB prevents the transition from juvenile ovary to testis and promotes ovarian development in zebrafish
All Figures: Figures for Pradhan et al., 2012

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Fig. 1 FIGURE 1. Heat-killed bacteria activate NF-κB and inflammatory genes in vitro. nZFL cells were treated with heat-killed E. coli (5 × 10⁶, 1× 10⁷, and 5 × 10⁷ cfu/ml), and the luciferase activity was determined after 12 h of exposure (A). ZFL cells were exposed to heat-killed E. coli (1× 10⁷, 5 × 10⁷, and 1× 10⁸ cfu/ml) for 24 h, and qPCR analysis was performed to determine p65 (B), nfkb1/p50 (C), ptgs2a (D), il8 (E) and tnfa (F). One-way analysis of variance was performed to determine statistical significant difference (*, p < 0.05; **, p < 0.01; ***, p < 0.001). n = 4. Error bars represent the mean ± S.D.

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