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Fig. 6. Tspan18 knockdown inhibits angiogenesis in vitro. (A) Tspan18 knockdown inhibited migration of HUAEC in an in vitro wound-healing assay. Transfection reagents served as a negative control. The margin of the wound is demarcated transwelled lines. Three independent experiments were performed and quantified wound width is shown (normalized to 0 h width). (B) Tspan18 knockdown inhibited invasion of HUAEC in transwell invasion assay. Quantification of invaded cells is shown on the right. (C) Tspan18 knockdown inhibited HUAEC tube formation on Matrigel. Quantification of tube length and number of junctions is shown on the bottom. The experiments in A–C were repeated three times with similar results. Error bars: s.d., *P<0.01, **P<0.002. P values were calculated using a two-tailed Student's t-test.