ZFIN Image: Ni et al., 2021, Fig. 7

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Figure Caption

Fig. 7 Diagram showing zebrafish ELAVL1a truncation and their antibacterial activities and binding to LPS and LTA.

a Diagram showing zebrafish ELAVL1a truncation. b Antibacterial activity of rE_181–242 against M. luteus and E. coli. c, d, e Interaction of rE_181–242 (c), rE_1–180 (d) and rE_243–324 (e) with LTA and LPS revealed by ELISA. f The in vivo bioactivity of rE_1–180, rE_181–242, and rE_243–324. The early (8-cell stage) embryos were first microinjected with PBS, BSA, anti-β-actin antibody (AcAb), anti ELAVL1a antibody (ELAVL1aAb), rE_1–180, rE_181–242, rE_243–324 and rELAVL1a, then challenged by injection with live A. hydrophila. The development of the embryos was observed and the cumulative mortality rate was calculated at 24 h after injection. All data were expressed as mean values ± S.D. (n = 3). The data are from three independent experiments performed in triplicate. The bars represent the mean ± SD. The significance of the difference was determined by one-way ANOVA. *p < 0.05. AcAb, anti-β-actin antibody; ELAVL1aAb, mouse anti-ELAVL1a antibody.

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