ZFIN Image: Ni et al., 2021, Fig. 3

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Figure Caption

Fig. 3 Binding of rELAVL1a to Gram-positive and Gram-negative bacteria, LTA, LPS, and lipid A.

a, b Western blotting about interaction of rELAVL1a with Gram-positive (a) and Gram-negative (b) bacteria. Lane M, marker; lane 1, purified rELAVL1a; lane 2, 4 and 6, M. luteus, B. subtilis and S. aureus (a) or E. coli, V. anguillarum and A. hydrophila (b) incubated in the presence of rELAVL1a; lane 3, 5, and 7, M. luteus, B. subtilis and S. aureus (a) or E. coli, V. anguillarum and A. hydrophila (b) incubated in the absence of rELAVL1a. c, d, e Interaction of rELAVL1a with LTA (c), LPS (d), Lipid A (e) revealed by ELISA. f Effects of various sugars on the interaction of rELAVL1a with LPS. g, h Interaction of rELAVL1a with LPS was inhibited by LTA (g) and LPS (h), the final concentration of rELAVL1a was 25 μg/ml. Each point in the graph represents the mean ± S.D. (n = 3). The data are from three independent experiments performed in triplicate. The bars represent the mean ± S.D.

Acknowledgments

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