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Fig. 3 Figure 3. Phagocytosis Defect Is Unique to tet2/3DM Embryonic Neutrophils (A–D) Representative images of the CHT region in pHrodo-red-injected 3-dpf wild-type, tet2−/−/, tet3−/−, and tet2/3DM mpx:GFP embryos. Scale bars represent 200 μm. (E) Quantification of phagocytosed beads by fluorescent area within GFP+ neutrophils in pixels squared. (F) Quantification of lysosome acidification by mean fluorescence intensity in arbitrary fluorescence units (A.F.U.). n = 5–7 embryos/condition, ∗∗p < 0.01, ∗∗∗∗p < 0.0001 by 2-way ANOVA with Tukey post hoc test of each condition compared to wild type.
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