ZFIN Image: Banks et al., 2021, Fig. 1

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Figure Caption

Fig. 1 Figure 1. Granulation Is Defective in tet3^−/− and tet2/3^DM Embryonic Neutrophils Representative images of neutrophil staining in wild-type, tet2^−/−, tet3^−/−, and tet2/3^DM embryos at 3 dpf. (A–D) Caudal hematopoietic tissue (CHT) region following WISH to analyze mpx transcripts. (E–H) CHT region of embryos stained for Sudan black (SB). Scale bars in (A)–(H) represent 200 μm. (I–L) High-resolution images of SB-stained neutrophils. Scale bars represent 2 μm. (M and N) Quantification of total mpx+ (immature and mature neutrophils) and SB+ (mature neutrophils) cells in embryos at 2 dpf (M) and 3 dpf (N); n = 10/condition, ∗p < 0.05, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001 by 2-way ANOVA with Tukey post hoc test.

Acknowledgments

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