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Figure 5 The effects of nicotine (NIC), 6-hydroxy-L-nicotine (6HLN), and cotinine (COT) (1 and 2 mg/L) administration in scopolamine (SCOP)-treated zebrafish on recognition memory evaluated within object discrimination task. (A) The locomotor tracking pattern of the zebrafish within the testing session of the object discrimination task according to the groups they belong to. The blue dot represents the beginning of the route, while the red dot represents the end of the route. The preference percentage (B) was considered the endpoint for recognition memory. The familiar and the novel objects were noted with F and N, respectively. The black dashed line (chance) indicates a 50% preference. The values are expressed as means ± S.E.M. (n = 10). ANOVA analysis revealed overall significant differences between groups for (B) F(8,81) = 8.078, p < 0.0001. For Tukey posthoc analyses—**** p < 0.0001, and ** p < 0.001.