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Fig. 2 Survival of ca17a+/+, ca17a+/−, and ca17a−/− zebrafish (Danio rerio) larvae over development. A: percent survival of larvae at 5 and 30 dpf under flow-through conditions. B: survival curves of larvae from 5 to 30 dpf under static conditions. There was a significant interaction of genotype and age on survival in A (two-way RM ANOVA; genotype × age, F = 57.6, P < 0.01; genotype, F = 57.6, P < 0.01; age, F = 2.0, P = 0.19; n = 5 tanks for ca17a+/+, n = 6 tanks for ca17a+/−, and n = 3 tanks for ca17a−/−). Bars with different letters are significantly different (P < 0.05) from one another. Survival curves in B were analyzed using Markov chain Monte Carlo sampler for multivariate generalized linear models (n = 8 tanks for ca17a+/+ and ca17a−/−, n = 7 tanks for ca17a+/−). *Significant difference from the wild-type ca17a+/+. Data are presented as means ± SE. dpf, days postfertilization.