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Figure 1 Gene expression analysis of crp1–7 in tissues of healthy zebrafish. The expression of crp1–7 was determined by RT-qPCR by using specific primers for each isoform (Table S1). ef1a mRNA was used as the endogenous control to normalize data, which are represented as the mean relative expression level × 103 ± SD of four different individuals. Statistical differences (p < 0.05, one-way ANOVA) between tissues are represented by: a (different from up to 1–2 tissues), b (different from up to 3–4 tissues), and c (different from up to 5–6 tissues). Data in bar graphs are summarized in a final double gradient colormap (descending blue gradient for values from 0 to 1 and ascending red gradient from values from 1 to ≥750).