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Figure 5 Expression of (a) Glut4 in zebrafish muscle tissues by immunofluorescence. Analysis of (b) Glut4, (c) AMPK, and (d) Akt signaling pathway in zebrafish muscle tissues by western blotting. The muscle extract was analyzed by western bottling, and the signal intensities were examined by the Fusion FX7 acquisition system (Vilber Lourmat, Eberhardzell, Germany). Experiments were performed in triplicate, and the data are expressed as mean± SE, n = 4 per group. ,#Values having different superscripts are significantly different at *p<0.05, **p<0.01, ***p<0.001, and ****p<0.0001 compared with the no sample-treated group; ##p<0.01 and ####p<0.0001 compared with the nontreated group. N.S.: no significance compared with the BAPTA-AM group.