Fig. 4 Fig. 4. DHA-S disturbed normal metabolism. A and B, The levels of glucose and lactate in H9c2 cell cultural medium supernatant after 24?h exposure of DHA-S at 1?g/L and compared with raw cultural medium.(n?=?3, data are represented as mean ± SEM, *, P?0.05; and ***, P?0.001, analyzed by Student?s unpaired T test.) C, the relative abundance of amino acids in H9c2 cell culture supernatant compared with height intensity of internal standard. (n?=?4, data are represented as mean.) D, the content of triacylglycerol (TG), total cholesterol (T-CHO) and low-density lipoprotein cholesterol (LDL-C) were examined in zebrafish larvae after 24?h exposure of DHA-S at 50?mg/L. (n?=?3, each group containing 50 embryos. Data are represented as mean ± SEM. ***, P?0.001, analyzed by Student?s unpaired T test.) E, the dose dependent protective effect of sodium bicarbonate on zebrafish from pericardial edema induced by DHA-S (100?mg/L). (n?=?3, each group containing 25 embryos) Data are represented as mean ± SEM. Representative pictures of DHA-S induced pericardial edema. Scale bar, 500?µm.
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