Fig. 2 Fig. 2. DHA-S induced Ca2+ deficiency in zebrafish. A, The total whole body Ca2+ content in zebrafish after 24?h exposure (48?72 hpf) with or without 50?mg/L DHA-S and exogenous addition of Ca2+. Data were analyzed by one-way analysis of variance (ANOVA) test (F (3, 8)?=?77.56; P?0.0001). B, The normal percent of zebrafish after 24?h exposure (48?72 hpf) with or without 50?mg/L DHA-S and exogenous addition of Ca2+. Data were analyzed by one-way analysis of variance (ANOVA) test (F (4, 10)?=?8.299; P?=?0.0032). C, The levels of active 1,25(OH)2D3 (calcitriol, CAT) in zebrafish with or without 50?mg/L DHA-S for 24?h (48?72 hpf) and exogenous addition of CAT. Data were analyzed by one-way analysis of variance (ANOVA) test (F (3, 8) =?66.17; P?0.0001). D, Relative mRNA levels (to Rpl13) of ion-regulated and vitamin D3-related genes. Data were analyzed by two-tailed, unpaired Student's t-test. In A, B, C and D, n?=?3, each group containing more than 35 larvae. All the data are expressed as mean ± SEM. *, P?0.05; and **, P?0.01; ***?, P?0.001; and ****, P?0.0001; untreated control versus treated groups.
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