Fig. 1 Fig. 1. DHA-S induces cardiovascular toxicity in zebrafish larvae. A, Representative pictures of DHA-S induced cerebral hemorrhage, mandibular dysplasia and pericardial edema in zebrafish during the exposure. B, Images of O-dianisidine-stained globin (representing erythrocytes) in zebrafish heart after 24?h of exposure (48?72?h post-fertilization) in water or 50?mg/L DHA-S. Right, quantification of DHA-S-treated and control groups. C, Heartbeat per minute of 10 zebrafish recorded and quantified. The difference between DHA-S-treated group and control group was analyzed by Student?s unpaired T test. D, Images of acridine orange-stained zebrafish larvae demonstrating apoptosis in hearts. The red dashed line indicates the cardiac region. Right, pooled data for the AO fluorescence intensity in whole zebrafish. In B and D groups, scale bar, 100?µm; n?=?3, each group containing 20 embryos. All the data are expressed as mean ± SEM. *, P?0.05; and **, P?0.01; analyzed by Student?s unpaired T test. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
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