Fig. 1

Fig. 1 Fig. 1. Maternal and zygotic pnrc2 promotes proper her1 and dlc expression. Wild-type (WT), zygotic pnrc2^oz22 (Zpnrc2), maternal pnrc2^oz22 (Mpnrc2), and maternal-zygotic pnrc2^oz22 (MZpnrc2) mutant embryos were raised to mid-segmentation stage (16?18 hpf) and probed for her1 (A?D) and dlc expression (E?F) by in situ hybridization (n ?? ?7 each). WT, Mpnrc2 mutant, and MZpnrc2 mutant embryos (n ?= ?10 per biological replicate) were analyzed by qPCR using primers to amplify across exon-exon boundaries to detect spliced her1 (I) and dlc (J) transcripts or primers to amplify across intron-exon boundaries to detect her1 (K) and dlc (L) unspliced transcripts. MZpnrc2 mutant embryos have ~4-fold higher levels of spliced her1 and dlc mRNA than wild-type or Mpnrc2 mutant embryos, which have comparable levels (I and J). Both MZpnrc2 and Mpnrc2 mutant embryos have ~2-fold less unspliced her1 and dlc transcripts compared to WT embryos (K and L). hpf ?= ?hours post-fertilization.