Fig. 1

Fig. 1 Fig. 1. Behavioral changes in adult zebrafish waterborne exposed to 0.3 and 3 μg/L glyphosate for 2 weeks. (A) Behavioral parameters assessed in standard 6-min novel tank test (NTT), as well as a cartoon of the experimental tank divided into two equal virtual zones, top and bottom; (B) Behavioral parameters assessed in standard 6-min dark-light test (DLT), as well as a cartoon of the experimental tank divided into two equal zones, white and black; (C) Behavioral parameters of zebrafish shoaling behavior in control and glyphosate-treated zebrafish. Data reported as scatter plot with the median (n = 23–29 for NTT, n = 20–22 for DLT and n = 19–20 for shoaling test), *p < 0.05, **p < 0.01, ***p < 0.001; one-way ANOVA with Dunnett’s multiple comparison test (Total distance, Distance in top, Time in top, Transitions to top, Erratic bouts, High mobility frequeny, Average interfish distance and Farthest distance) or Kruskal Wallis test with Bonferroni correction (Latency to the top, Erratic duration) Data from 2 to 4 independent experiments.