(A) Experimental design for MEP glial EdU incorporation assay. (B) Lateral view of a nkx2.2a:nls-eGFP larva showing EdU+/GFP+/Sox10+ MEP glia at 72 hpf (outlined arrowheads). (C) Percentage of EdU+ MEP glia per motor nerve at 72 hpf; n = 89 MEP glia in 10 larvae. (D) Distribution of the number of MEP glia per motor nerve at 4 dpf; n = 214 MEP glia and. n = 60 motor nerves in 20 larvae. (E) Lateral view of a sox2:egfp larvae at 55 hpf showing sox2+ MEP glia (white outlined arrowhead). Yellow dashed lines denote the edge of the spinal cord. Scale bar, (B) 20 µm, (E) 10 µm.
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