(A) Lateral view of the trunk of a foxd3:mcherry;nkx2.2a:mcerulean embryo showing nkx2.2a+/foxd3+ MEP glia (outlined arrowheads) and nkx2.2a+/foxd3- perineurial glia (arrowheads) at MEP TZs at 50 hpf. (B) Percentage of MEP glia versus perineurial glia that exit the neural tube first; n = 52 motor nerves. (C) Lateral view of a motor exit point in an olig2:dsred; nkx2.2a:megfp embryo showing nkx2.2a+/olig2+ MEP glia dividing (outlined arrowheads) at 55 hpf. (D) In situ hybridization of olig2+ cells in the spinal cord at 48 and 72 hpf. Dashed lines denote the edge of the spinal cord and asterisks denote the DRG. Scale bar (A) 25 µm, (C) 20 µm, (D) 10 µm.
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