Figure 7—figure supplement 1.

Figure 7—figure supplement 1.

(A) Representative micrographs of zebrafish tailbud localization of DHB-mSC. (B) Representative images of the tailbud of control or 50 µM palbociclib treated embryos (n ≥ 3 embryos). (C) Quantification of DHB in the posterior forming somite of control or 50 µM palbociclib treated embryos at 20–22 somite stage. (D) Percentage of cell in G1 in the posterior forming somite of control or 50 µM palbociclib treated embryos. (E) DHB-mSc expression in primitive red blood cells in the intermediate cell mass at 24 hpf. Insets show exclusion of DHB from the nucleus indicating G2. (F) Quantification of DHB ratio suggests that these cells are in G2 with a mean ratio of 3.00. Line and error bars depict mean ± SD. (G, H) Representative micrographs of DHB-mSC in the posterior forming somite (G) and differentiated muscle (H). (I) Quantification of DHB ratio in cycling cells (posterior somite) and quiescent cells (muscle). Line and error bars depict mean ± SD. (J, K) Representative images of DHB-mSC in the NPCs (J) and in the quiescent epidermis (K). (L) Quantification of DHB ratio confirms the NPCs are in G1 with a mean ratio of 0.33. Line and error bars depict mean ± SD. In panels B, C, F, H, I, K, and L, insets, orange box, are zoom-ins. Scale bar = 20 µm.