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Fig. 4 Figure 4. Ythdf2 and Ythdf3 Are Redundantly Required for Zebrafish Ovary Development (A) Numbers of male and female fish of each genotype. Sibling control and double ythdf2;ythdf3 homozygotes were offspring from the same cross, depicted on top. (B) Gonad histology of double homozygous (ythdf2−/−;ythdf3−/−) and sibling control fish from the cross in (A). At 27 dpf, mutants exhibit less developed juvenile ovaries than controls. At 35 dpf, 6 sibling fish had adult ovaries and 8 had testes, while all 12 ythdf2−/−;ythdf3−/− fish had testes. I, stage I oocytes; II, stage II oocytes; triangle, apoptotic oocyte; sg, spermatogonia; sc, spermatocytes. n, replicate number with similar gonads. Scale bars, 40 μm. (C) Numbers of male and female fish of each genotype, following treatment with 17α-ethynylestradiol (EE2). Fish were from the same cross as in (A).