ZFIN Image: Turan et al., 2020, Fig. 5

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Figure Caption

Fig. 5 Figure 5. LITAF down-regulates total NEDD4-2 expression. Adenovirus for expression of GFP (control) or LITAF-HA was incubated with 3wRbCM (10 MOI) (A) or NRbCM (2 MOI) (B) for 48 h. HEK cells were transfected with plasmid encoding GFP (control) or HA-tagged LITAF (C) for 48 h. Representative Western blotting data from respective extracts to measure expression of NEDD4-2, tubulin, and LITAF-HA. D, NEDD4-2–FLAG, LITAF-HA, and tubulin expression in HEK cells transfected with expression plasmids for NEDD4-2–FLAG, LITAF-HA, or control plasmid for 48 h. E, Respective relative LITAF-dependent NEDD4-2 expression levels normalized to tubulin expression from four independent experiments performed in duplicate (Student's t test; p < 0.05). In the scatter plot, averaged values for the four independent experiments together with the means and S.E. values are shown.

Acknowledgments

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