Figure 5

Figure 5

The VSR rapidly decreases over time in synj1 mutants. (A) Impaired balance in synj1^Q296X mutants. The blue arc-shape arrow indicates the direction of the current. Yellow arrows indicate wild type siblings and white arrowheads indicate synj1^Q296X mutants. (B,C) Representative tracking trace of a wild type sibling and a synj1^Q296X larva. Note the tail movements during the initial cycles and then cessation of the reflex. (D–F) The maximum tail angle, normalized integral, and percentage of time above threshold show no significant difference during the initial cycles, but then dramatically decrease in synj1^Q296X larvae. (G) The number of active cycles (default threshold was 5°) were reduced in synj1 mutants compared to wild type siblings. (H) The half time of the VSR in synj1^Q296X larvae was significantly less compared to wild type siblings. Mean ± SEM for each genotype was plotted and a two-way ANOVA with Benjamini-Hochberg correction was performed. *p < 0.05; **p < 0.01; and ***p < 0.001.