The VSR in zebrafish larvae at 5 days post-fertilization (5 dpf). (A) Infrared (IR) imaging acquisition system, which contains a rotary platform driven by a motor, an objective (10x), an IR LED beneath the chamber holder, and an IR camera. The white dotted box indicates the mounting chamber and chamber holder (B). (B) Diagram of a vertically mounted larva in the chamber unit. The larval head is embedded in 2% low melting point agarose and the trunk and pectoral fins are free to move in the E3 media. (C,D) The test device provides a yaw stimulus at 0.53 Hz and rotation of the head ±75°. (E) Still frames of four contiguous cycles of the evoked tail responses in a wild type larva are shown. Asterisks indicate black frames.
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