ZFIN Image: Li et al., 2020, Fig. 2

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Figure Caption

Fig. 2 gata5 is a target gene of miR-92. The expression of mCherry was observed as red fluorescence at 12 h after injection of one-cell zygotes of zebrafish. Sample numbers for each group were about 100 zygotes. (A–H) Embryos were injected with reporters containing either the full-length gata5 3′ UTR (full) or a construct in which MRE(s) were deleted (D1: MRE 1 deleted; D2: MRE 2 deleted; D12: MRE 1 and MRE 2 deleted). (A–D) Reporter plasmids were injected alone. (E–H) Reporter plasmids were co-injected with miR-92. (I) The proportion of fluorescent embryos in (A–D). (J) The proportion of fluorescent embryos in (E–H). Fluorescence-positive and -negative embryos are depicted in red and blue, respectively.

Acknowledgments

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