Fig 2

Fig 2

Macrophage recruitment at 6 hours post-injection (hpi) with egg antigens. (A) Macrophages recruited to SEA from WT or omega-1 knockout eggs (Δω1). (B) Macrophages recruited to SEA or omega-1. (C) Mean macrophage recruitment to omega-1 for each of four experiments. Individual experiments represented with unique symbols; triangles and squares represent means of panels B and D, respectively. (D) Macrophages recruited to omega-1 or DEPC-treated omega-1. (E) Macrophages recruited to WT or RNase mutant omega-1. All omega-1 injections were performed using 0.02 ng of plant-expressed omega-1, with the exception of (E) which used HEK-expressed WT or mutant omega-1 injected at a 5-fold higher concentration of 0.1 ng to compensate for lack of LeX glycosylation in plant-expressed and natural omega-1. Statistics, ANOVA with Dunnett’s post-test comparing all samples to PBS (B) or WT (A,E); (D) non-parametric ANOVA with Dunn’s post-test comparing all samples to omega-1; (C) paired t-test. All horizontal bars, means. Statistics; * p<0.05, ** p<0.01, and *** p<0.001.